Veratox® for Aflatoxin M1

Item No. 8019

  • Gold standard in mycotoxin testing
  • Cost effective microwell format
  • Ideal for batch testing
  • Fast, accurate, and easy to use
  • Broad range of validated and approved commodities
BRAND Neogen Co.
MADE IN USA
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PRODUCT DETAILS

Veratox® for Aflatoxin M1 is intended for the quantitative analysis of aflatoxin M1 in commodities such as milk, milk powder, butter and cheese.

Specifications

Analyte Aflatoxin M1
Brand Veratox®
Cross Reactivity
  • Aflatoxin M1 100%
  • Aflatoxins B1, B2, G1, G2, and M2 <1%
Limit of Detection 4.3 ppt
Package Dimensions 10.00 IN x 8.00 IN x 2.40 IN
Package Weight 0.20 LB
Quantity per Package 38 tests

  1. Microwell holder
  2. 96 antibody-coated microwells
  3. 48 red-marked mixing wells
  4. 6 yellow-labeled bottles of 0, 5, 15, 30, 60, and 100 ppt aflatoxin controls
  5. 1 bottle of sample diluent
  6. 1 bottle of 25x wash buffer. Each bottle is enough to prepare 1 L in distilled or deionized water (pH 7.4)
  7. 2 blue-labeled bottles of aflatoxin-HRP conjugate solution
  8. 1 green-labeled bottle of K-Blue® Substrate solution
  9. 1 red-labeled bottle of Red Stop solution
  1. Scale capable of weighing 1–11 grams
  2. Microwell reader with a 650 nm filter (Neogen® items 9301, 9302, 9303)
  3. 12-channel pipettor (Neogen item 9273)
  4. 100 µL pipettor (Neogen item 9272)
  5. Tips for 100 µL and 12-channel pipettors (Neogen item 9410)
  6. Paper towels or equivalent absorbent material
  7. Plastic bucket for use as waste receptacle
  8. Plate rotary shaker
  9. Timer (Neogen item 9426)
  10. Vortex
  11. Waterproof marker
  12. Wash bottle (Neogen item 9400)
  13. 3 reagent boats for 12-channel pipettor (blue, green and red-labeled) (Neogen item 9435)
  14. Centrifuge with rotor for 50 cc tubes
  15. 50 cc tubes with caps
  16. Distilled or deionized water
  17. Dichloromethane (cheese testing only)
  18. Hexanes (cheese testing only)
  19. 100% methanol (cheese testing only)
  20. Chemical fume hood (cheese testing only)
  21. High-speed blender (cheese testing only)
  22. Grater (cheese testing only)
  23. Water bath evaporator with nitrogen gas stream (cheese testing only)
  24. Incubator capable of maintaining 50°C (cheese testing only)
  25. Glass tube for evaporation (cheese testing only)

Veratox for Aflatoxin M1 is a competitive enzyme-linked immunosorbent assay (ELISA) that allows the user to obtain exact concentrations of aflatoxin M1 in dairy products in parts per trillion (ppt). Free aflatoxin M1 in the samples and controls is added to microwells that have been coated with anti-aflatoxin M1 monoclonal antibody. A wash step will remove any unbound aflatoxin.

During a second incubation, an aflatoxin-HRP conjugate will bind to the remaining free binding site on the antibody. After a wash step, substrate is added and allowed to react with the bound conjugate to produce blue color. More blue color means less aflatoxin M1. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of aflatoxin M1.

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