Reveal 2.0 for DSP
Item No. 9561
- Simple extraction and testing procedure
- Comparable with FDA and EU Commission permitted levels
- Rapid results easily determined by reader
PRODUCT DETAILS
Reveal® 2.0 for DSP (diarrhetic shellfish poisoning) is a single step lateral flow assay that detects okadaic acid (OA) and dinophysistoxin (DTX) derivatives. These marine biotoxins can accumulate in shellfish during toxic algal blooms. Once the toxin is present in the shellfish tissue it cannot be destroyed by heat during cooking. The E.U. and U.S. have established maximum threshold levels of 160 ppb.
Specifications
| Analyte | DSP |
| Brand | Reveal® |
| Limit of Detection | 160 ppb |
| Matrix | Shellfish |
| Package Dimensions | 8.40 IN x 5.40 IN x 2.25 IN |
| Package Weight | 0.80 LB |
| Platform | Lateral Flow |
| Quantity per Package | 24 tests |
| Result Type | Qualitative |
| Testing Time | 15.00 Minutes |
| Unit Analyte | DSP |
Reveal 2.0 for DSP is a single-step lateral flow device based on a competitive immunoassay format. In summary, the shellfish extract is wicked through a reagent zone, containing antibodies specific for OA-group toxins that have been conjugated to colored particles. If the toxins are present in the sample, the toxin will be captured by the particle-antibody complex. The complex then is wicked onto a membrane, which contains a stationary capture zone of a toxin-protein conjugate. This zone captures any uncomplexed toxin particle-antibody. Therefore, as the concentration of toxins in the sample increases, the test line intensity decreases. The membrane also contains a stationary control zone which always will form regardless of the level of toxins.
MATERIALS PROVIDED
- 24 Reveal 2.0 for DSP lateral flow test strips
- 24 wells
- 24 vials of DSP buffer A (clear cap)
- 24 vials of DSP buffer B (red cap)
- 25 extraction bags
- 48 disposable 100 µL pipettors
MATERIALS REQUIRED BUT NOT PROVIDED
- Marine Biotoxins Starter Kit (Neogen® item 9563)
- Microwell holder
- 1 roller
- 1 bag clip (white clip and green straw)
- DSP Hydrolysis Pack (Neogen item 9554)
- 2.5 M sodium hydroxide (NaOH) solution (5 mL)
- 2.5 M hydrochloric acid (HCl) solution (5 mL)
- 24 glass sample vials
- Distilled water
- Methanol (analytical grade recommended, VWR 20864.320)
- Filter syringes (Neogen item 9420)
- Sample collection cups with lids (Neogen items 9428, 9428B)
- Blender (Neogen items 9493, 9477 or 9495)
- Scale capable of weighing 0.5–400 g ± 0.1 g (Neogen item 9427)
- Timer (Neogen item 9452)
- Graduated cylinder, 50 mL (Neogen item 9447)
- Sample collection tubes with caps, 5 mL (Neogen item 9421, 9421B)
- Pipettor, 100–1000 mL, and pipette tips (Neogen item 9463, 9464)
- Heater block (capable of holding 76 ± 2°C (Recommended VWR 460-3250 & 460-3280)
- AccuScan Pro reader (Neogen item 9565)
Training
Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.
SEAFOOD TEST KITS
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CONTACT US
You can contact our ABP sales engineers for detailed information about our products.
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