Agri-Screen for Fumonisin
Item No. 8810
- Rapid and easy onsite testing
- Screen at various levels
- No reader required
- Broad range of validated and approved commodities
PRODUCT DETAILS
Agri-Screen® for Fumonisin is a competitive direct ELISA that provides a visible screening result against a known control of fumonisin in such commodities as barley, corn, DDGS, milo, popcorn, rice, soybeans and wheat.
Specifications
| Analyte | Fumonisin |
| Brand | Agri-Screen® |
| Cross Reactivity | Total fumonisins (B1, B2, B3) |
| Limit of Detection | 5 ppm |
| Package Dimensions | 10.50 IN x 8.00 IN x 3.50 IN |
| Package Weight | 0.70 LB |
| Prop 65 Warning | WARNING: This product can expose you to chemicals including fumonisin B1, which is known to the State of California to cause cancer, and thimerosal and methanol, which are known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov. |
| Quantity per Package | 12 tests |
| Result Type | Qualitative |
| Testing Time | 15.00 Minutes |
- 24 antibody-coated microwells
- 24 red-marked mixing wells
- 1 yellow-labeled bottle of 5 ppm fumonisin control (see precautions for handling of methanol solution)
- 1 blue-labeled bottle of fumonisin-HRP conjugate solution
- 1 green-labeled bottle of K-Blue® Substrate solution
- 1 red-labeled bottle of Red Stop solution
- 14 pink-labeled bottles for sample dilution, prefilled with 7.9 mL of deionized water (Dilution bottles not provided with Neogen item #8811)
- Directions for use
- Extraction materials (items 4 through 6 available in kit form from Neogen, item #8052):
- 70% methanol solution (Neogen item #8055)
- Distilled or deionized water
- 250 mL graduated cylinder (Neogen item #9368)
- Container with 125 mL capacity (Neogen item #9428)
- Neogen filter syringes, Whatman #1 filter paper, or equivalent (Neogen item #9420/#9430)
- Sample collection tubes (Neogen item #9421)
- 50 mL graduated cylinder (Neogen item #9447)
- High-speed blender (Neogen item #9493/#9477)
- Agri-Grind grinder or equivalent (Neogen item #9401/#9453)
- Scale capable of weighing 5-25 grams (Neogen item #9427)
- Pipettor 100 µL (Neogen item #9272/#9278)
- Tips for 100 µL pipettor (Neogen item #9410/#9407)
- Paper towels or equivalent absorbent material
Procedures
First, samples and controls are added to their respective test wells. Next, an enzyme conjugate (target substance chemically linked with an enzyme) is added. The samples/controls and conjugate are mixed and transferred to antibody wells where they compete for the antibody binding sites. The more target substance in the sample, the less conjugate that binds in the wells.
After an incubation, the wells are washed to remove all unbound materials.
A substrate, which changes color in the presence of the conjugate, is added to the wells. During an incubation, blue color develops in proportion to the amount of conjugate versus target substance in the wells. The more conjugate bound, the more blue color that develops, indicating less substance present.
Results are read visually in a screening format—the less blue color, or more red, the more target substance detected.
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