Alert® for Sulfites
Item No. 9500
- Simple three step method
- Rapid time to results
- Visual interpretation, no equipment
PRODUCT DETAILS
Sulfiting agents, sodium bisulfite and sodium metabisulfite, are used with shrimp, lobster and related crustaceans to prevent melanosis (a.k.a. “blackspot”). While sulfiting agents are very beneficial to the seafood industry, some consumers are highly allergic to sulfite residues in food. For this reason, FDA regulations state that seafood containing sulfite residues of 10 parts per million (ppm) or more must be labeled as containing sulfites. Monitoring and verification of sulfite residues are required as part of a Hazard Analysis and Critical Control Points (HACCP) program. Alert® for Sulfites is a very simple and rapid semi-quantitative test for detecting sulfite residues in shrimp.
Specifications
| Analyte | Sulfites |
| Brand | Alert® |
| Limit of Detection | 10 ppm |
| Package Dimensions | 8.00 IN x 4.00 IN x 1.50 IN |
| Package Weight | 0.10 LB |
| Platform | ELISA |
| Quantity per Package | 200 tests |
| Result Type | Semi-Quantitative |
| Testing Time | 60.00 Seconds |
MATERIALS PROVIDED
- 1 orange-capped bottle of Activator Solution
- 1 blue-capped bottle of Dye Reagent
- Color Chart
Each test kit contains antibody-coated microwells with antibodies specific to the kit’s target substance.
First, samples and controls are added to their respective test wells. Next, an enzyme conjugate (target substance chemically linked with an enzyme) is added. The samples/controls and conjugate are mixed and transferred to antibody wells where they compete for the antibody binding sites. The more target substance in the sample, the less conjugate that binds in the wells.
After an incubation, the wells are washed to remove all unbound materials.
A substrate, which changes color in the presence of the conjugate, is added to the wells. During an incubation, blue color develops in proportion to the amount of conjugate versus target substance in the wells. The more conjugate bound, the more blue color that develops, indicating less substance present.
Results are read visually in a screening format—the less blue color, or more red, the more target substance detected. In a quantitative format, results are obtained by measuring the wells’ color change in a microwell reader and comparing the readings against a standard curve.
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