Reveal® Q+ for DON

Item No. 8385

  • Easy to use Lateral Flow format
  • For use with portable reader with data management software
  • Rapid and accurate quantitative testing
  • FGIS and AOAC approved method
BRAND Neogen Co.
MADE IN USA
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PRODUCT DETAILS

Reveal® Q+ for DON is a single-step lateral flow immunochromatographic assay based on a competitive immunoassay format intended for the quantitative testing of DON in grain and grain products.

Specifications

Analyte Deoxynivalenol (DON)
Approvals FGIS: 2018-106; AOAC-PTM 071901
Brand Reveal®
Limit of Detection 0.3 ppm
Package Dimensions 8.30 IN x 5.20 IN x 2.40 IN
Package Weight 0.55 LB
Platform Lateral Flow
Quantity per Package 25 tests
Range of Quantitation 0.30 ppm - 6.00 ppm
Result Type Quantitative
Testing Time 3.00 Minutes

  1. 25 Reveal Q+ for DON test strips
  2. 25 red sample dilution cups
  3. 25 sample cups
  4. 2 bottles of sample diluent
  5. Instructions for use

  1. Distilled or deionized water
  2. Sample collection cups with lids (Neogen® item 9428)
  3. Agri-Grind grinder or equivalent (Neogen item 9401, 9453)
  4. Scale capable of weighing 5–50 g ± 0.1 g (Neogen item 9427)
  5. Timer (Neogen item 9426)
  6. Reveal sample cup rack (Neogen item 9475)
  7. Dispensing pump or graduated cylinder (Neogen item 9448, 9447)
  8. Filter syringe or Whatman no. 1 filter (Neogen item 9420, 9430)
  9. Sample collection tubes with caps (Neogen item 9421, 9421B)
  10. Pipettor, 100 µL (Neogen item 9860)
  11. Pipette tips, 100 µL (Neogen item 9407, 9410, 9417)
  12. Pipettor, 100-1000 µL (Neogen item 9463)
  13. Pipette tips, 100–1000 µL (Neogen item 9464, 9487)
  14. Reveal AccuScan Gold reader or AccuScan Pro reader
  15. Raptor Integrated Analysis System (Neogen item 9680 or 9696)
  16. Raptor cartridges (Neogen item 9681)

Assay Principle

Reveal Q+ for DON is a single-step lateral flow immunochromatographic assay based on a competitive immunoassay format. The extract is wicked through a reagent zone, which contains antibodies specific for DON conjugated to colloidal gold particles. If DON is present, it will be captured by the particle-antibody complex. The DON-antibody-particle complex then is wicked onto amembrane, which contains a zone of DON conjugated to a protein carrier. This zone captures any uncomplexed DON antibody, allowing the particles to concentrate and form a visible line. As the level of DON in a sample increases, free DON will complex with the antibody-gold particles. This, in turn, allows less antibody-gold to be captured in the test zone. Therefore, as the concentration of DON in the sample increases, the test line density decreases. Algorithms programmed into the AccuScan® reader convert these line densities into a quantitative result displayed in parts per million (ppm). The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of DON, ensuring the strip is functioning properly.

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