Reveal® Q+ MAX for Aflatoxin

Item No. 8088

  • Common MAX 1 aqueous extraction
  • Precise, reproducible mycotoxin results in just 6 minutes
  • FGIS and AOAC approved method
BRAND Neogen Co.
MADE IN USA
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PRODUCT DETAILS

Easy, rapid and usable anywhere

Reveal® Q+ MAX for Aflatoxin is a lateral flow test that offers results in just 6 minutes. This line of mycotoxin tests can be used for six mycotoxins using a common, water-based extraction, which eliminates the need for hazardous materials in the extraction process. It can be used on a variety of commodities, including wheat and corn. When paired with a NEOGEN reader, testers can easily read, analyze and store precise, reproducible, quantitative results.

Specifications

Analyte Aflatoxin
Approvals FGIS 2019-125; AOAC-PTM 071801
Brand Reveal®
Cross Reactivity
  • B1 100%
  • B2 91%
  • G1 87%
  • G2 64%
Limit of Detection 3 ppb
Package Dimensions 7.40 IN x 4.80 IN x 3.90 IN
Package Weight 1.00 LB
Platform Lateral Flow
Quantity per Package 25 tests
Range of Quantitation 3.00 ppb - 50.00 ppb
Result Type Quantitative
Testing Time 6.00 Minutes

  1. 25 Reveal Q+ MAX for Aflatoxin test strips
  2. 25 red sample dilution cups
  3. 25 clear sample cups
  4. 1 bottle of sample diluent
  5. 25 MAX 1 Aqueous Extraction packets
  6. Instructions for use

  1. Extraction Materials
    1. Sample collection cups with lids 125 mL (Neogen item 9428, 9428B)
    2. Sample collection tubes with caps (Neogen item 9421, 9421B)
    3. Filter syringes, Whatman #4 filter paper or equivalent (Neogen item 9420, 9429, 9519) OR
    4. Centrifuge, mini (Neogen item 9330)
    5. Microcentrifuge tubes (Neogen item 9172)
    6. Dispensing pump or graduated cylinder (Neogen item 9448, 9447)
  2. Agri-Grind grinder or equivalent (Neogen item 9401,9453)
  3. Scale capable of weighing 5–50 grams ± 0.1 g (Neogen item 9427)
  4. Timer (Neogen item 9426,9452)
  5. Reveal sample cup rack (Neogen item 9475)
  6. Pipettor, 100 μL (Neogen item 9860, 9290)
  7. Pipette tips, 1-200 μL (Neogen item 9407, 9410, 9417)
  8. Distilled or deionized water
  9. AccuScan Gold reader (Neogen item 9595) or AccuScan Pro reader
  10. MAX 1 Aqueous Extraction packets (Neogen item 8089)
  11. MAX 1–GIPSA Aqueous Extraction packets (Neogen item 8089G)

Assay Principle

Reveal Q+ MAX for Aflatoxin is a single-step lateral flow immunochromatographic assay based on a competitive immunoassay format. The extract is wicked through a reagent zone, which contains antibodies specific for aflatoxin conjugated to colloidal gold particles. If aflatoxin is present, it will be captured by the particle-antibody complex. The aflatoxin-antibody-particle complex then is wicked onto a membrane, which contains a zone of aflatoxin conjugated to a protein carrier. This zone captures any uncomplexed aflatoxin antibody, allowing the particles to concentrate and form a visible line. As the level of aflatoxin in a sample increases, free aflatoxin will complex with the antibody-gold particles. This allows less antibody-gold to be captured in the test zone. Therefore, as the concentration of aflatoxin in the sample increases, the test line density decreases. Algorithms programmed into the AccuScan® readers convert these line densities into a quantitative result displayed in parts per billion (ppb). The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of aflatoxin, ensuring the strip is functioning properly.

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