Reveal Q+ for Fumonisin

Item No.  8885

  • For use with portable reader with data management software
  • Rapid and accurate quantitative testing
BRAND Neogen Co.
MADE IN USA
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PRODUCT DETAILS

Reveal® Q+ for Fumonisin is a single-step lateral flow immunochromatographic assay based on a competitive immunoassay format intended for the quantitative testing of fumonisin in grain and grain products.

Specifications

Analyte Fumonisin
Approvals FGIS: 2018-116
Brand Reveal®
Limit of Detection 0.3 ppm
Package Dimensions 8.30 IN x 5.20 IN x 2.40 IN
Package Weight 0.44 LB
Platform Lateral Flow
Quantity per Package 25 tests
Range of Quantitation 0.30 ppm - 6.00 ppm
Result Type Quantitative
Testing Time 6.00 Minutes

  1. 65% ethanol solution (Neogen® item 8073, 8074)
  2. Sample collection cups with lids (Neogen item 9428)
  3. Agri-Grind grinder or equivalent (Neogen item 9401, 9453)
  4. Scale capable of weighing 5–50 g ± 0.1 g (Neogen item 9427)
  5. Timer (Neogen item 9426)
  6. Reveal sample cup rack (Neogen item 9475)
  7. AccuScan reader: AccuScan III (Neogen item 9590), AccuScan Pro (Neogen item 9565), or AccuScan Gold (Neogen item 9595)
  8. Dispensing pump or graduated cylinder (Neogen item 9448, 9447)
  9. Filter syringe or Whatman no.1 filter (Neogen item 9420, 9430)
  10. Sample collection tubes with caps (Neogen item 9421, 9421B)
  11. Pipettor, 100 µL (Neogen item 9272, 9278)
  12. Pipette tips, 100 µL (Neogen item 9407, 9410, 9417)
  13. Pipettor, 200 µL (Neogen item 9488)
  14. Pipette tips, 200–1000 µL (Neogen item 9464, 9487)

  1. 25 Reveal Q+ for Fumonisin test strips
  2. 25 red sample dilution cups
  3. 25 clear sample cups
  4. 1 bottles of sample diluent
  5. Instructions for use

Assay Principle

Reveal Q+ for Fumonisin is a single-step lateral flow immunochromatographic assay based on a competitive immunoassay format. The extract is wicked through a reagent zone, which contains antibodies specific for fumonisin conjugated to colloidal gold particles. If fumonisin is present, it will be captured by the particle-antibody complex. The fumonisin-antibody-particle complex then is wicked onto a membrane, which contains a zone of fumonisin conjugated to a protein carrier. This zone captures any uncomplexed fumonisin antibody, allowing the particles to concentrate and form a visible line. As the level of fumonisin in a sample increases, free fumonisin will form a complex with the antibody-gold particles. This allows less antibody-gold to be captured in the test zone. Therefore, as the concentration of fumonisin in the sample increases, the test line density decreases. Algorithms programmed into the AccuScan® readers convert these line densities into a quantitative result displayed in parts per million (ppm). The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line always will form regardless of the presence of fumonisin, ensuring the strip is functioning properly.

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