Reveal Q+MAX for T-2/HT-2 is a single-step lateral flow immunoassay. In the test, the extract is wicked through the reagent zone, which contains antibodies specific for T-2/HT-2 conjugated to colloidal gold particles (gold complex). If T-2/HT-2 is present, it will be captured by the gold complex. The gold complex, along with any free gold-complex, is then wicked onto a membrane, which contains a zone of T-2/HT-2 conjugated to a protein carrier. This zone captures any unbound gold complex, allowing the particles to concentrate and form a visible line. As the level of T-2/HT-2 in a sample increases, free T-2/HT-2 will bind with the gold complex allowing less gold complex to be captured in the test zones. Therefore, as the concentration of T-2/HT-2 in the sample increases, the test line density decreases. Algorithms programmed into the AccuScan^® readers convert the line intensities into a quantitative result displayed in parts per billion (ppb). The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of T-2/HT-2, ensuring the strip is functioning properly.