Reveal® Q+ MAX for Zearalenone

Item No. 8188

  • Common MAX 1 aqueous extraction
  • Precise, reproducible mycotoxin results in just 5 minutes
  • Can detect a wide range (25–500 parts per billion) of zearalenone
BRAND Neogen Co.
MADE IN USA
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PRODUCT DETAILS

Easy, rapid and usable anywhere

Reveal® Q+ MAX for Zearalenone is a lateral flow test that offers results in just 5 minutes. This line of mycotoxin tests can be used for six mycotoxins using a common, water-based extraction, which eliminates the need for hazardous materials in the extraction process. It can be used on a variety of commodities, including wheat and corn. When paired with a NEOGEN reader, testers can easily read, analyze and store precise, reproducible, quantitative results.

Specifications

Analyte Zearalenone
Brand Reveal®
Limit of Detection Corn: 36 ppb Wheat: 21 ppb
Package Dimensions 7.40 IN x 4.90 IN x 3.90 IN
Package Weight 1.10 LB
Platform Lateral Flow
Quantity per Package 25 tests
Range of Quantitation 25.00 ppb - 500.00 ppb
Result Type Quantitative
Testing Time 5.00 Minutes
  1. 25 Reveal Q+ MAX for Zearalenone test strips
  2. 25 red conical sample dilution cups
  3. 25 clear sample cups
  4. 2 bottles of red-labeled sample diluent
  5. 1 bottle of yellow-labeled dilution buffer (for samples > 800 ppb)
  6. 25 MAX 1 Aqueous Extraction packets
  7. Instructions for use
  1. Extraction Materials
    1. Sample collection cups with lids 125 mL (Neogen item 9428, 9428B)
    2. Sample collection tubes with caps (Neogen item 9421, 9421B)
    3. Filter syringes, Whatman #4 filter paper or equivalent (Neogen item 9420, 9429, 9519) OR
    4. Dispensing pump or graduated cylinder (Neogen item 9448, 9447)
  2. Agri-Grind grinder or equivalent (Neogen item 9401, 9453)
  3. Scale capable of weighing 5–50 grams ± 0.1 g (Neogen item 9427)
  4. Timer (Neogen item 9426, 9452)
  5. Reveal sample cup rack (Neogen item 9475)
  6. Pipettor, 100 µL (Neogen item 9860, 9290)
  7. Pipettor 100 — 1000 µL (Neogen item 9463)
  8. Pipette tips, 1-200 µL (Neogen item 9407, 9410, 9417)
  9. Distilled or deionized water
  10. AccuScan Gold reader (Neogen item 9595) or AccuScan Pro reader
  11. MAX 1 Aqueous Extraction packets (Neogen item 8089)
  12. MAX 1–GIPSA Aqueous Extraction packets (Neogen item 8089G)

Assay Principle

Reveal Q+ MAX for Zearalenone is a single-step lateral flow immunoassay. In the test, the extract is wicked through the reagent zone, which contains antibodies specific for zearalenone conjugated to colloidal gold particles (gold complex). If zearalenone is present, it will be captured by the gold complex. The gold complex, along with any free gold-complex, is then wicked onto a membrane, which contains a zone of zearalenone conjugated to a protein carrier. This zone captures any unbound gold complex, allowing the particles to concentrate and form a visible line. As the level of zearalenone in a sample increases, free zearalenone will bind with the gold complex allowing less gold complex to be captured in the test zones. Therefore, as the concentration of zearalenone in the sample increases, the test line density decreases. Algorithms programmed into the AccuScan® readers convert the line intensities into a quantitative result displayed in parts per billion (ppb). The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of zearalenone, ensuring the strip is functioning properly.

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