Veratox® for Aflatoxin

Item No. 8030

  • Cost effective microwell format ideal for batch testing
  • FGIS and AOAC approved method
  • Broad range of validated and approved commodities
BRAND Neogen Co.
MADE IN USA
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PRODUCT DETAILS

Veratox® for Aflatoxin is a competitive direct ELISA that provides a quantitative analysis of aflatoxin in grain and grain products.

Specifications

Analyte Aflatoxin
Approvals
  • USDA/GIPSA 2018-113
  • AOAC-RI 050901
Brand Veratox®
Cross Reactivity Total aflatoxins (B1, B2, G1, G2)
Limit of Detection 1.4 ppb
Package Dimensions 8.50 IN x 6.50 IN x 1.50 IN
Package Weight 0.40 LB
Platform ELISA
Prop 65 Warning
WARNING: This product can expose you to chemicals including Methanol which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 40 tests
Range of Quantitation 5.00 ppb - 50.00 ppb
Result Type Quantitative
Testing Time 5.00 Minutes

  1. 48 antibody-coated microwells
  2. 48 red-marked mixing wells
  3. 4 yellow-labeled bottles of 0, 5, 15 and 50 ppb aflatoxin controls (see precautions for handling of methanol solution)
  4. 1 blue-labeled bottle of aflatoxin-HRP conjugate solution
  5. 1 green-labeled bottle of K-Blue® Substrate solution
  6. 1 red-labeled bottle of Red Stop solution
  1. Extraction materials (items 3 through 5 available in kit form from Neogen®, item 8052):
    1. 70% ACS Grade methanol (Neogen item 8055, 8056)
    2. 250 mL graduated cylinder (Neogen item 9368)
    3. Container with 125 mL capacity (Neogen item 9428)
    4. Neogen filter syringes, Whatman #1 filter paper, or equivalent (Neogen item 9420, 9430)
    5. Sample collection tubes (Neogen item 9421)
  2. High-speed blender (Neogen item 9493, 9477)
  3. Agri-Grind grinder or equivalent (Neogen item 9401, 9453)
  4. Scale capable of weighing 5-50 grams (Neogen item 9427)
  5. Microwell reader with a 650 nm filter (Neogen item 9303)
  6. Pipettor, 12-channel (Neogen item 9273)
  7. Pipettor, 100 µL (Neogen item 9272, 9290)
  8. Pipette tips for 100 µL and 12-channel pipettors (Neogen item 9410, 9407, 9417)
  9. Paper towels or equivalent absorbent material
  10. Plastic bucket for use as waste receptacle
  11. Microwell holder (Neogen item 9402)
  12. Timer (Neogen item 9426)
  13. Waterproof marker
  14. Wash bottle (Neogen item 9400)
  15. 2 reagent boats for 12-channel pipettor (Neogen item 9450)
  16. Distilled or deionized water

Assay Principle

Veratox for Aflatoxin is a direct competitive ELISA in a microwell format, which allows the user to obtain exact concentrations in parts per billion (ppb). Free aflatoxin in the samples and controls is allowed to compete with enzyme-labeled aflatoxin (conjugate) for the antibody binding sites. After a wash step, substrate is added, which reacts with the bound conjugate to produce blue color. More blue color means less aflatoxin. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of aflatoxin.

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