Veratox® for DON 2/3

Item No. 8335

  • Cost effective microwell format ideal for batch testing
  • FGIS and AOAC approved method
  • Broad range of validated and approved commodities
BRAND Neogen Co.
MADE IN USA
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PRODUCT DETAILS

Veratox® for DON 2/3 is a competitive direct ELISA that provides a quantitative analysis of DON in cereal grains and grain products, such as wheat, wheat flour, wheat midds, wheat bran, corn, cornmeal, corn screenings, barley, malted barley and oats.

Specifications

Analyte Deoxynivalenol (DON)
Approvals
  • GIPSA 2018-111
  • AOAC-RI 090901
Brand Veratox®
Cross Reactivity DON 100%. Also cross-reactive with 3-acetyl DON but does not bind to other trichothecenes.
Limit of Detection 0.1 ppm
Package Dimensions 8.00 IN x 6.00 IN x 1.80 IN
Package Weight 0.40 LB
Platform ELISA
Prop 65 Warning
WARNING: This product can expose you to chemicals including pyridine, which is known in the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 38 tests
Range of Quantitation 0.50 ppm - 5.00 ppm
Result Type Quantitative
Testing Time 5.00 Minutes

  1. 48 antibody-coated microwells
  2. 48 red-marked mixing wells
  3. 5 yellow-labeled bottles of 0, 0.5, 1, 2 and 6 ppb aflatoxin controls
  4. 1 blue-labeled bottle of DON-HRP conjugate solution
  5. 1 green-labeled bottle of K-Blue® Substrate solution
  6. 1 red-labeled bottle of Red Stop solution

  1. Extraction materials
    1. Distilled or deionized water
    2. Graduated cylinder
    3. Container with 125 mL capacity (500 mL for GIPSA method) (Neogen® item 9428)
    4. Whatman no. 1 filter paper, Neogen filter syringe, or equivalent (Neogen items 9430, 9420)
    5. Sample collection tubes (Neogen item 9421)
  2. Agri-Grind grinder, or equivalent (Neogen item 9401, 9453)
  3. Scale capable of weighing 5-50 grams (Neogen item 9427)
  4. Microwell reader with a 650 nm filter (Neogen item 9303)
  5. Pipettor, 12-channel (Neogen item 9273)
  6. Pipettor, 100 µL (Neogen item 9272, 9290)
  7. Tips for 100 µL and 12-channel pipettors (Neogen item 9407, 9410, 9417)
  8. Paper towels or equivalent absorbent material
  9. Plastic bucket for use as waste receptacle
  10. Microwell holder (Neogen item 9402)
  11. Timer (Neogen item 9452, 9426)
  12. Waterproof marker
  13. Wash bottle (Neogen item 9400)
  14. 2 reagent boats for 12-channel pipettor (Neogen item 9435)
  15. Distilled or deionized water

Assay Principle

Veratox for DON 2/3 is a competitive direct enzyme-linked immunosorbent assay (CD-ELISA), which allows the user to obtain exact concentrations in parts per million (ppm). Free DON in the samples and controls is allowed to compete with enzyme-labeled DON (conjugate) for the antibody binding sites. After a wash step, substrate is added, which reacts with the bound conjugate to produce blue color. More blue color means less DON. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of DON.

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