Veratox® for DON 5/5
Item No. 8331
- Cost effective microwell format ideal for batch testing
- FGIS approved method
- Broad range of validated and approved commodities
PRODUCT DETAILS
Veratox® for DON 5/5 is a competitive direct ELISA that provides a quantitative analysis of DON in cereal grains and grain products, such as wheat, wheat flour, wheat midds, wheat bran, corn, cornmeal, corn screenings, barley, malted barley and oats.
Specifications
| Analyte | Deoxynivalenol (DON) |
| Approvals | FGIS 2018-110 |
| Brand | Veratox® |
| Cross Reactivity | DON 100%. Also cross-reactive with 3-acetyl DON but does not bind to other trichothecenes. |
| Limit of Detection | 0.1 ppm |
| Package Dimensions | 8.50 IN x 6.00 IN x 1.80 IN |
| Package Weight | 0.40 LB |
| Platform | ELISA |
| Prop 65 Warning | WARNING: This product can expose you to chemicals including pyridine, which is known in the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov. |
| Quantity per Package | 38 tests |
| Range of Quantitation | 0.50 ppm - 5.00 ppm |
| Result Type | Quantitative |
| Testing Time | 10.00 Minutes |
- 48 antibody-coated microwells
- 48 red-marked mixing wells
- 5 yellow-labeled bottles of 0, 0.5, 1, 2 and 6 ppb DON controls
- 1 blue-labeled bottle of DON-HRP conjugate solution
- 1 green-labeled bottle of K-Blue® Substrate solution
- 1 red-labeled bottle of Red Stop solution
- Extraction materials
- Distilled or deionized water
- Graduated cylinder
- Container with 125 mL capacity (500 mL for GIPSA method) (Neogen® item 9428)
- Whatman no. 1 filter paper, Neogen filter syringe, or equivalent
- Sample collection tubes (Neogen item 9421)
- Agri-Grind grinder, or equivalent (Neogen item 9401, 9453)
- Scale capable of weighing 5-50 grams (Neogen item 9427)
- Microwell reader with a 650 nm filter (Neogen item 9303)
- Pipettor, 12-channel (Neogen item 9273)
- Pipettor, 100 µL (Neogen item 9272, 9290)
- Tips for 100 µL and 12-channel pipettors (Neogen item 9407, 9410, 9417)
- Paper towels or equivalent absorbent material
- Plastic bucket for use as waste receptacle
- Microwell holder (Neogen item 9402)
- Timer (Neogen item 9452, 9426)
- Waterproof marker
- Wash bottle (Neogen item 9400)
- 2 reagent boats for 12-channel pipettor (Neogen item 9435)
- Distilled or deionized water
Assay Principle
Veratox for DON 5/5 is a competitive direct enzyme-linked immunosorbent assay (CD-ELISA), which allows the user to obtain exact concentrations in parts per million (ppm). Free DON in the samples and controls is allowed to compete with enzyme-labeled DON (conjugate) for the antibody binding sites. After a wash step, substrate is added, which reacts with the bound conjugate to produce blue color. More blue color means less DON. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of DON.
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CONTACT US
You can contact our ABP sales engineers for detailed information about our products.
ABP from 1985 to the present;
It represents world-leading brands and is the leading company for the technology needs of the Grain, Flour, Food, Feed industry.
