Veratox® HS for DON
(High Sensitivity)

Item No. 8332

  • Quantitative results for lower levels of mycotoxins
  • Cost effective microwell format
  • Ideal for batch testing
  • Fast, accurate, and easy to use
BRAND Neogen Co.
MADE IN USA
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PRODUCT DETAILS

Veratox® HS for DON is a competitive direct ELISA that provides a quantitative analysis of lower levels of DON in cereal grains and grain products, such as wheat, wheat flour, wheat midds, wheat bran, corn, cornmeal, corn screenings, barley, malted barley, oats and processed cereal.

Specifications

Analyte Deoxynivalenol (DON)
Brand Veratox®
Cross Reactivity DON 100%. Also cross-reactive with 3-acetyl DON but does not bind to other trichothecenes
Limit of Detection 25 ppb
Package Dimensions 4.80 IN x 2.70 IN x 6.40 IN
Package Weight 0.50 LB
Platform ELISA
Prop 65 Warning
WARNING: This product can expose you to chemicals including pyridine, which is known in the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 38 tests
Range of Quantitation 25.00 ppb - 250.00 ppb
Result Type Quantitative
Testing Time 20.00 Minutes

  1. 48 antibody-coated microwells
  2. 48 red-marked mixing wells
  3. 5 yellow-labeled bottles of 0, 25, 50, 100 and 250 ppb DON controls
  4. 1 blue-labeled bottle of DON-HRP conjugate solution
  5. 1 green-labeled bottle of K-Blue® Substrate solution
  6. 1 red-labeled bottle of Red Stop solution

  1. Extraction materials:
    1. Distilled or deionized water
    2. Graduated cylinder (Neogen® item #9447/#9368)
    3. Container with 125 mL capacity (Neogen item #9428)
    4. Whatman #1 filter paper, Neogen filter syringe, or equivalent (Neogen item #9430/#9420)
    5. Sample collection tubes (Neogen item #9421)
  2. Agri-Grind grinder, or equivalent (Neogen item #9401)
  3. Scale capable of weighing 5-50 grams (Neogen item #9427)
  4. Microwell reader with a 650 nm filter (Neogen item #9302)
  5. Pipettor, 12-channel (Neogen item #9273)
  6. Pipettor, 100 µL (Neogen item #9272)
  7. Tips for 100 µL and 12-channel pipettors (Neogen item #9410/#9407)
  8. Paper towels or equivalent absorbent material
  9. Plastic bucket for use as waste receptacle
  10. Microwell holder (Neogen item #9402)
  11. Timer (Neogen item #9426)
  12. Waterproof marker
  13. Wash bottle (Neogen item #9400)
  14. 2 reagent boats for 12-channel pipettor (Neogen item #9435)
  15. Distilled or deionized water

Assay Principle

Veratox HS for DON is a competitive direct enzyme-linked immunosorbent assay (CD-ELISA) which allows the user to obtain exact concentrations in parts per billion (ppb). Free DON in the samples and controls is allowed to compete with enzyme-labeled DON (conjugate) for the antibody binding sites. After a wash step, substrate is added, which reacts with the bound conjugate to produce blue color. More blue color means less DON. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of DON.

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