Veratox® for Fumonisin is a competitive direct ELISA that provides a quantitative analysis of fumonisin in commodities such as barley, corn, DDGS, milo, popcorn, rice, soybeans and wheat.
Specifications
Analyte
Fumonisin
Approvals
AOAC Official Method 2001.06
Brand
Veratox®
Cross Reactivity
Total fumonisins (B1, B2, B3)
Limit of Detection
0.2 ppm
Package Dimensions
5.30 IN x 4.50 IN x 8.10 IN
Package Weight
2.20 LB
Platform
ELISA
Prop 65 Warning
WARNING: This product can expose you to chemicals including fumonisin B1, which is known to the State of California to cause cancer, and thimerosal and methanol, which are known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
5 yellow-labeled bottles of 0, 1, 2, 4 and 6 ppm fumonisin controls (see Precautions for handling methanol solution)
1 blue-labeled bottle of fumonisin-HRP conjugate solution
1 green-labeled bottle of K-Blue® Substrate solution
1 red-labeled bottle of Red Stop solution
1 dilution kit that contains 40 dilution bottles prefilled with 7.9 mL of a 10% methanol/water solution (dilution bottles not provided with Neogen item 8831)
Agri-Grind grinder or equivalent (Neogen item 9401/9453)
Scale capable of weighing 2–25 grams ±0.1g (Neogen item 9427)
Microwell reader with a 650 nm filter (Neogen item 9303)
Pipettor, 12-channel (Neogen item 9273)
Pipettor, 100 µL (Neogen item 9272, 9290)
Tips for 100 µL and 12-channel pipettors (Neogen item 9410, 9407, 9417)
Paper towels or equivalent absorbent material
Plastic half gallon bucket for use as waste receptacle
Microwell holder (Neogen item 9402)
Timer (Neogen item 9426)
Waterproof marker
Wash bottle (Neogen item 9400)
2 reagent boats for 12-channel (Neogen item 9450)
Distilled or deionized water
Assay Principle
Veratox for Fumonisin allows the user to obtain exact concentrations in parts per million (ppm). Free fumonisin in the samples and controls is allowed to compete with enzyme-labeled fumonisin (conjugate) for the antibody binding sites. After a wash step substrate is added, which reacts with the bound conjugate to produce blue color. More blue color means less fumonisin. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of fumonisin.
