Veratox® for Ochratoxin is a competitive direct ELISA that provides a quantitative analysis of ochratoxin in such commodities as corn, barley, wheat, green coffee, oats, rice, soybeans and various dried fruits.
Specifications
Analyte
Ochratoxin
Brand
Veratox®
Cross Reactivity
Ochratoxin A 100%
Ochratoxin B 18%
Limit of Detection
1 ppb
Package Dimensions
8.50 IN x 6.00 IN x 1.60 IN
Package Weight
0.40 LB
Platform
ELISA
Prop 65 Warning
WARNING: This product can expose you to chemicals including ochratoxin A, which is known to the State of California to cause cancer, and thimerosal and methanol, which are known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
Agrigrind grinder or equivalent (Neogen item 9401, 9453)
Scale capable of weighing 5–50 g (Neogen item 9427)
Microwell reader with a 650 nm filter (Neogen item 9303)
Pipettor, 12-channel (Neogen item 9273)
Pipettor, 100 µL (Neogen item 9272, 9290)
Pipette tips for 100 µL and 12-channel pipettors (Neogen item 9410, 9407, 9417)
Paper towels or equivalent absorbent material
Plastic bucket for use as waste receptacle
Microwell holder (Neogen item 9402)
Timer (Neogen item 9426)
Waterproof marker
Wash bottle (Neogen item 9400)
2 reagent boats for 12-channel pipettor (Neogen item 9450)
Distilled or deionized water
Assay Principle
Veratox for Ochratoxin is a competitive direct enzyme-linked immunosorbent assay (CD-ELISA) which allows the user to obtain exact concentrations in parts per billion (ppb). Free ochratoxin in the samples and controls is allowed to compete with enzyme-labeled ochratoxin (conjugate) for the antibody binding sites. After a wash step, substrate is added which reacts with the bound conjugate to produce blue color. More blue color means less ochratoxin. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of ochratoxin.
