Veratox® HS for Ochratoxin
(High Sensitivity)
Item No. 8632
- Quantitative results for lower levels of mycotoxins
- Cost effective microwell format
- Ideal for batch testing
- Fast, accurate, and easy to use
PRODUCT DETAILS
Veratox® HS for Ochratoxin is a competitive direct ELISA that provides quanitative analysis of lower levels of ochratoxin in wheat and corn.
Specifications
| Analyte | Ochratoxin |
| Brand | Veratox® |
| Limit of Detection | 1 ppb |
| Package Dimensions | 8.00 IN x 6.00 IN x 2.20 IN |
| Package Weight | 0.30 LB |
| Platform | ELISA |
| Prop 65 Warning | WARNING: This product can expose you to chemicals including ochratoxin A, which is known to the State of California to cause cancer, and methanol, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov. |
| Quantity per Package | 38 tests |
| Range of Quantitation | 2.00 ppb - 10.00 ppb |
| Result Type | Quantitative |
| Testing Time | 30.00 Minutes |
- 48 antibody-coated microwells
- 48 red-marked mixing wells
- 48 green-marked sample dilution mixing wells
- 5 yellow-labeled bottles of 0, 1, 3, 7.5, and 15 ppb ochratoxin standard controls (see precautions for handling of methanol solutions)
- 1 white-labeled bottle of corn sample diluent solution (for corn samples only)
- 1 blue-labeled bottle of ochratoxin-HRP conjugate solution
- 1 green-labeled bottle of K-Blue® Substrate solution
- 1 red-labeled bottle of Red Stop solution
- Directions for use
- Extraction materials (items 3 through 5 available in kit form from Neogen®, item 8052):
- ACS grade 70% methanol (Neogen item 8055)
- 250 mL graduated cylinder (Neogen item 9368)
- Container with 125 mL capacity (Neogen item 9428)
- Whatman no. 1 filter paper, or equivalent (Neogen item 9430)
- Sample collection tubes (Neogen item 9421)
- Agrigrind grinder or equivalent (Neogen items 9401, 9453)
- Scale capable of weighing 5–50 g (Neogen item 9427)
- Microwell reader with a 650 nm filter (Neogen item 9303)
- Pipettor, 12-channel (Neogen item 9273)
- Pipettor, 100 µL (Neogen items 9272, 9290)
- Pipette tips for 100 µL and 12-channel pipettors (Neogen items 9410, 9407, 9417)
- Paper towels or equivalent absorbent material
- Plastic bucket for use as waste receptacle
- Microwell holder (Neogen item 9402)
- Timer (Neogen item 9426)
- Waterproof marker
- Wash bottle (Neogen item 9400)
- 2 reagent boats for 12-channel pipettor (Neogen item 9450)
- Distilled or deionized water
Assay Principle
Veratox HS for Ochratoxin is a competitive direct enzyme-linked immunosorbent assay (CD-ELISA) which allows the user to obtain exact concentrations in parts per billion (ppb). Free ochratoxin in the samples and controls is allowed to compete with enzyme-labeled ochratoxin (conjugate) for the antibody binding sites. After a wash step, substrate is added which reacts with the bound conjugate to produce blue color. More blue color means less ochratoxin. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of ochratoxin.
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