Veratox for T-2/HT-2
Item No. 8230
- Cost effective microwell format
- Ideal for batch testing
- Fast, accurate, and easy to use
- Broad range of validated and approved commodoties
PRODUCT DETAILS
Veratox® for T-2/HT-2 is a competitive direct ELISA that provides a quantitative analysis of T-2 toxin and HT-2 toxin in commodities such as wheat, corn, barley, oats and rye.
Specifications
| Analyte | T-2/HT-2 |
| Brand | Veratox® |
| Cross Reactivity |
|
| Limit of Detection | 25 ppb |
| Package Dimensions | 8.50 IN x 6.00 IN x 2.00 IN |
| Package Weight | 0.40 LB |
| Platform | ELISA |
| Prop 65 Warning | WARNING: This product can expose you to chemicals including methanol, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov. |
| Quantity per Package | 38 tests |
| Range of Quantitation | 25.00 ppb - 250.00 ppb |
| Result Type | Quantitative |
| Testing Time | 10.00 Minutes |
- 48 antibody-coated microwells
- 48 red-marked mixing wells
- 5 yellow-labeled bottles of 0, 25, 50, 100 and 250 ppb T-2 toxin controls (see precautions for handling of methanol solution)
- 1 blue-labeled bottle of HT-2 toxin-HRP conjugate solution
- 1 green-labeled bottle of K-Blue® Substrate solution
- 1 red-labeled bottle of Red Stop solution
- Directions for use
- Extraction materials (items 3 through 5 are available in kit form, Neogen® item 8052):
- 70% methanol (ACS Grade) (Neogen item 8055, 8056)
- 250 mL graduated cylinder (Neogen item 9368)
- Container with 125 mL capacity (Neogen item 9428)
- Neogen filter syringes, Whatman no. 1 filter paper, or equivalent (Neogen items 9420, 9430)
- Sample collection tubes (Neogen item 9421)
- Agri-Grind grinder or equivalent (Neogen item 9401, 9453)
- Scale capable of weighing 2–25 grams (Neogen item 9427)
- Microwell reader with a 650 nm filter (Neogen item 9303)
- Pipettor, 12-channel (Neogen item 9273)
- Pipettor, 100 µL (Neogen item 9272, 9290)
- Pipette tips for 100 µL and 12-channel pipettors (Neogen items 9410, 9407, 9417)
- Paper towels or equivalent absorbent material
- Plastic bucket for use as waste receptacle
- Microwell holder (Neogen item 9402)
- Timer (Neogen item 9426)
- Waterproof marker
- Wash bottle (Neogen item 9400)
- 2 reagent boats for 12-channel pipettor (Neogen item 9450)
- Distilled or deionized water
Assay Principle
Veratox for T-2/HT-2 is a competitive direct ELISA in a microwell format that allows the user to obtain exact concentrations in parts per billion (ppb) of either T-2, HT-2 or a combination of both. Free T-2 or HT-2 toxins in the samples and controls are allowed to compete with enzyme-labeled HT-2 toxin (conjugate) for the antibody binding sites. After a wash step substrate is added, which reacts with the bound conjugate to produce blue color. More blue color means less T-2/HT-2 toxins. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of T-2/HT-2 toxins.
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You can contact our ABP sales engineers for detailed information about our products.
ABP from 1985 to the present;
It represents world-leading brands and is the leading company for the technology needs of the Grain, Flour, Food, Feed industry.
