Veratox for T-2/HT-2

Item No. 8230

  • Cost effective microwell format
  • Ideal for batch testing
  • Fast, accurate, and easy to use
  • Broad range of validated and approved commodoties
BRAND Neogen Co.
MADE IN USA
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PRODUCT DETAILS

Veratox® for T-2/HT-2 is a competitive direct ELISA that provides a quantitative analysis of T-2 toxin and HT-2 toxin in commodities such as wheat, corn, barley, oats and rye.

Specifications

Analyte T-2/HT-2
Brand Veratox®
Cross Reactivity
  • T-2 toxin 100%
  • HT-2 toxin B 100%
Limit of Detection 25 ppb
Package Dimensions 8.50 IN x 6.00 IN x 2.00 IN
Package Weight 0.40 LB
Platform ELISA
Prop 65 Warning
WARNING: This product can expose you to chemicals including methanol, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 38 tests
Range of Quantitation 25.00 ppb - 250.00 ppb
Result Type Quantitative
Testing Time 10.00 Minutes

  1. 48 antibody-coated microwells
  2. 48 red-marked mixing wells
  3. 5 yellow-labeled bottles of 0, 25, 50, 100 and 250 ppb T-2 toxin controls (see precautions for handling of methanol solution)
  4. 1 blue-labeled bottle of HT-2 toxin-HRP conjugate solution
  5. 1 green-labeled bottle of K-Blue® Substrate solution
  6. 1 red-labeled bottle of Red Stop solution
  7. Directions for use

  1. Extraction materials (items 3 through 5 are available in kit form, Neogen® item 8052):
    1. 70% methanol (ACS Grade) (Neogen item 8055, 8056)
    2. 250 mL graduated cylinder (Neogen item 9368)
    3. Container with 125 mL capacity (Neogen item 9428)
    4. Neogen filter syringes, Whatman no. 1 filter paper, or equivalent (Neogen items 9420, 9430)
    5. Sample collection tubes (Neogen item 9421)
  2. Agri-Grind grinder or equivalent (Neogen item 9401, 9453)
  3. Scale capable of weighing 2–25 grams (Neogen item 9427)
  4. Microwell reader with a 650 nm filter (Neogen item 9303)
  5. Pipettor, 12-channel (Neogen item 9273)
  6. Pipettor, 100 µL (Neogen item 9272, 9290)
  7. Pipette tips for 100 µL and 12-channel pipettors (Neogen items 9410, 9407, 9417)
  8. Paper towels or equivalent absorbent material
  9. Plastic bucket for use as waste receptacle
  10. Microwell holder (Neogen item 9402)
  11. Timer (Neogen item 9426)
  12. Waterproof marker
  13. Wash bottle (Neogen item 9400)
  14. 2 reagent boats for 12-channel pipettor (Neogen item 9450)
  15. Distilled or deionized water

Assay Principle

Veratox for T-2/HT-2 is a competitive direct ELISA in a microwell format that allows the user to obtain exact concentrations in parts per billion (ppb) of either T-2, HT-2 or a combination of both. Free T-2 or HT-2 toxins in the samples and controls are allowed to compete with enzyme-labeled HT-2 toxin (conjugate) for the antibody binding sites. After a wash step substrate is added, which reacts with the bound conjugate to produce blue color. More blue color means less T-2/HT-2 toxins. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of T-2/HT-2 toxins.

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