Veratox® for Total Milk
Item No. 8470
- Accurate and easy-to-use
- Quantitate or screen samples
- High specificity
PRODUCT DETAILS
Veratox for Total Milk Allergen is used for the quantitative analysis of milk residue in food products and clean-in-place rinses. Can also be used to screen environmental swabs
Specifications
| Analyte | Milk |
| Brand | Veratox® |
| Limit of Detection | <1 ppm NFDM |
| Package Dimensions | 10.50 IN x 8.00 IN x 2.80 IN |
| Package Weight | 1.10 LB |
| Platform | ELISA |
| Prop 65 Warning | WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov. |
| Quantity per Package | 38 tests |
| Range of Quantitation | 2.50 ppm NFDM - 25.00 ppm NFDM |
| Result Type | Quantitative |
| Testing Time | 30.00 Minutes |
- 48 antibody-coated microwells (24 per pouch)
- 48 red-marked transfer wells (24 per pouch)
- 5 yellow-labeled bottles of 0, 2.5, 5, 10 and 25 ppm nonfat dry milk (NFDM) controls
- 4 blue-labeled bottles of enzyme-labeled antibody conjugate
- 1 green-labeled bottle of K-Blue® Substrate
- 1 red-labeled bottle of Red Stop solution
- 5 foil pouches of 10 mM PBS dry powder extraction solvent; each pouch contains enough powder to prepare 1 L of extraction solvent
- 2 wide-mouth bottles of 40 mL PBS-Tween wash buffer concentrate; each bottle contains enough concentrate to prepare 1 L of wash buffer
- 50 g of extraction additive in a specimen cup
- Plastic scoop to measure extraction additive
- Allergen Extraction Kit (Neogen item 8429)
- 20 disposable plastic extraction bottles
- 20 disposable transfer pipettes
- Shaker water bath adjusted to 60°C ± 1° (140°F) with clamps to hold extraction bottles
- Adjustable 50-200 µL pipettor (Neogen item 9276)
- Pipette tips (Neogen item 9410)
- 12-channel pipettor (Neogen item 9273)
- Timer (Neogen item 9426)
- Microwell strip reader with a 650 nm filter (Neogen item 9303)
- 1 L bottle to prepare washing solution (Neogen item 9472)
- 1 L heat safe bottle to prepare extract solution (Neogen item 9472)
- Paper towels or equivalent absorbent material
- Microwell holder (Neogen item 9402)
- Waterproof marker
- Wash bottle (Neogen item 9400)
- Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
- Scale capable of weighing 5 g ± 0.1 g (Neogen item 9427)
- 3 reagent multichannel pipettor boats (Neogen item 9435)
- Distilled or deionized water
Veratox for Total Milk Allergen is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Milk protein residue is extracted from samples with a buffered salt solution (Phosphate Buffered Saline, or PBS) by shaking in a heated water bath. Milk protein residue is extracted from samples and added to antibody-coated wells (capture antibody) where it binds to the antibody during an incubation. Any unbound protein residue is washed away and a second antibody (detector antibody), which is enzyme labeled, is added. The detector antibody binds to the already bound milk protein residue. After a second wash, the substrate is added. Color develops as a result of the presence of bound detector antibody. Red Stop reagent is added and the color of the resulting solution is observed. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of allergen.
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