Veratox® for Zearalenone

Item No. 8110

  • Cost effective microwell format
  • Ideal for batch testing
  • Fast, accurate, and easy to use
  • Broad range of validated and approved commodoties
BRAND Neogen Co.
MADE IN USA
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PRODUCT DETAILS

Veratox® for Zearalenone is a competitive direct ELISA that provides a quantitative analysis of zearalenone in such commodities as corn, wheat, barley and DDGS.

Specifications

Analyte Zearalenone
Brand Veratox®
Cross Reactivity
  • Zearalenone 100%, α-zearalenol 73%
  • β-zearalenol 23%, Zearalanone 63%
  • α-zearalanol 36%, β-zearalanol 15%
Limit of Detection 5 ppb
Package Dimensions 8.50 IN x 6.00 IN x 2.00 IN
Package Weight 0.40 LB
Platform ELISA
Prop 65 Warning
WARNING: This product can expose you to chemicals including methanol and thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 38 tests
Range of Quantitation 25.00 ppb - 500.00 ppb
Result Type Quantitative
Testing Time 10.00 Minutes

  1. 48 antibody-coated microwells
  2. 48 red-marked mixing wells
  3. 6 yellow-labeled bottles of 0, 25, 75, 150, 300 and 500 ppb zearalenone controls
  4. 1 blue-labeled bottle of zearalenone-HRP conjugate solution
  5. 1 green-labeled bottle of K-Blue® Substrate solution
  6. 1 red-labeled bottle of Red Stop solution
  7. Directions for use

  1. Extraction materials (items 3 through 5 are available in kit form from Neogen®, item 8052):
    1. 70% ACS Grade methanol (Neogen item 8055, 8056)
    2. 250 mL graduated cylinder (Neogen item 9368)
    3. Container with 125 mL capacity (Neogen item 9428)
    4. Neogen filter syringes, Whatman #1 filter paper, or equivalent (Neogen item 9420, 9430)
    5. Centrifuge, Mini (Neogen item 9330)
    6. Micro-centrifuge tube (Neogen item 9172)
    7. Sample collection tubes (Neogen item 9421)
  2. Agri-Grind grinder or equivalent (Neogen item 9401, 9453)
  3. Scale capable of weighing 5–50 grams (Neogen item 9427)
  4. Microwell reader with a 650 nm filter (Neogen item 9303)
  5. Pipettor, 12-channel (Neogen item 9273)
  6. Pipette tips for 12 channel pipettor (Neogen item 9407, 9410, 9417)
  7. Pipettor, 100–1000 µL (Neogen item 9290)
  8. Pipette tips for 100–1000 µL 12-channel pipettors (Neogen item 9410, 9407, 9417)
  9. Paper towels or equivalent absorbent material
  10. Plastic bucket for use as a waste receptacle
  11. Microwell holder (Neogen item 9402)
  12. Timer (Neogen item 9426)
  13. Wash bottle (Neogen item 9400)
  14. 2 reagent boats for 12-channel pipettor (Neogen item 9450)
  15. Waterproof marker
  16. Distilled or deionized water

Assay Principle

Veratox MAX for Zearalenone is a competitive direct ELISA in a microwell format which allows the user to obtain exact concentrations in parts per billion (ppb). Free zearalenone in the samples and controls is allowed to compete with enzyme-labeled zearalenone (conjugate) for the antibody binding sites. After a wash step substrate is added, which reacts with the bound conjugate to produce blue color. More blue color means less zearalenone. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of zearalenone.

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