Veratox® HS for Fumonisin is a competitive direct ELISA that provides a quantitative analysis of fumonisin in commodities such as barley, corn, DDGS, milo, popcorn, rice, soybeans and wheat.
Specifications
Analyte
Fumonisin
Brand
Veratox®
Cross Reactivity
Total fumonisins (B1, B2, B3)
Limit of Detection
50 ppb
Package Dimensions
8.50 IN x 6.00 IN x 1.75 IN
Package Weight
0.50 LB
Platform
ELISA
Prop 65 Warning
WARNING: This product can expose you to chemicals including fumonisin B1, which is known to the State of California to cause cancer, and thimerosal and methanol, which are known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
Agri-Grind grinder or equivalent (Neogen item #9401/#9453)
Scale capable of weighing 2-25 g + 0.1 g (Neogen item #9427)
Microwell reader with a 650 nm filter (Neogen item #9302)
Pipettor, 12-channel (Neogen item #9273)
Pipettor, 100 µL (Neogen item #9272)
Tips for 100 µL and 12-channel pipettors (Neogen item #9410/#9407)
Paper towels or equivalent absorbent material
Plastic 1/2 gallon bucket for use as waste receptacle
Microwell holder (Neogen item #9402)
Timer (Neogen item #9426)
Waterproof marker
Wash bottle (Neogen item #9400)
2 reagent boats for 12-channel pipettor (Neogen item #9435)
Distilled or deionized water
10–15 mL containers/bottles (Neogen item #9474)
Assay Principle
Veratox HS for Fumonisin allows the user to obtain exact concentrations in parts per billion (ppb). Free fumonisin in the samples and controls is allowed to compete with enzyme-labeled fumonisin (conjugate) for the antibody binding sites. After a wash step substrate is added, which reacts with the bound conjugate to produce blue color. More blue color means less fumonisin. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of fumonisin.
