Veratox® HS MAX for Total Aflatoxin (High Sensitivity)

Item No. 8032

  • Aqueous extraction - no harsh solvents
  • Quantitative results for lower levels of mycotoxins
  • Cost effective microwell format
  • Ideal for batch testing
  • Fast, accurate, and easy to use
BRAND Neogen Co.
MADE IN USA
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PRODUCT DETAILS

Veratox® HS MAX for Total Aflatoxin is a competitive direct ELISA that provides a quantitative analysis of lower levels of aflatoxin. This test is designed to detect the four principle types of Aflatoxin B1, B2, G1 and G2 with superior cross reactivity when compared to other available kits. Veratox HS for Total Aflatoxin utilizes an aqueous extraction procedure eliminating the need for harsh solvents in your laboratory.

Specifications

Specifications
Analyte Aflatoxin
Brand Veratox®
Cross Reactivity Total aflatoxins (B1, B2, G1, G2)
Limit of Detection 0.8 ppb
Package Dimensions 7.30 IN x 4.90 IN x 4.00 IN
Package Weight 1.20 LB
Platform ELISA
Quantity per Package 38 tests
Range of Quantitation 1.00 ppb - 10.00 ppb
Result Type Quantitative
Testing Time 30.00 Minutes

  1. 48 antibody-coated microwells
  2. 48 red-marked mixing wells
  3. 5 yellow-labeled bottles of 0, 1, 3, 5 and 10 ppb aflatoxin controls
  4. 1 blue-labeled bottle of aflatoxin-HRP conjugate solution
  5. 1 green-labeled bottle of K-Blue® Substrate solution
  6. 1 red-labeled bottle of Red Stop solution
  7. 25 MAX 2 Aqueous Extraction Packets
  8. 1 pink-labeled bottle of dilution diluent

  1. Extraction materials (items 3 through 5 available in kit form from Neogen®, item 8052):
    1. MAX 2 Aqueous Extraction Packets (Neogen item 8036)
    2. 250 mL graduated cylinder (Neogen item 9368)
    3. Container with 125 mL capacity (Neogen item 9428)
    4. Neogen Filter syringes, Whatman #4 filter paper, or equivalent
    5. Sample collection tubes (Neogen item 9421)
  2. Agri-Grind grinder or equivalent (Neogen item 9401)
  3. Scale capable of weighing 5-25 grams (Neogen item 9427)
  4. Microwell reader with a 650 nm filter (Neogen item 9301, 9302)
  5. Pipettor, 12-channel (Neogen item 9273)
  6. Pipettor, 100 µL (Neogen item 9272)
  7. Pipette tips for 100 µL and 12-channel pipettors (Neogen item 9410)
  8. Paper towels or equivalent absorbent material
  9. Plastic bucket for use as waste receptacle
  10. Microwell holder (Neogen item 9402)
  11. Timer (Neogen item 9426)
  12. Waterproof marker
  13. Wash bottle (Neogen item 9400)
  14. 2 reagent boats for 12-channel pipettor (Neogen item 9435)
  15. Distilled or deionized water
  16. Shaker, Rockit (Neogen item 9390)
  17. Plate Shaker (i.e. IKA MS3 digital shaker)

Assay Principle

Veratox HS MAX for Total Aflatoxin is a direct competitive ELISA in a microwell format which allows the user to obtain exact concentrations in parts per billion (ppb). Free aflatoxin in the samples and controls is allowed to compete with enzyme-labeled aflatoxin (conjugate) for the antibody binding sites. After a wash step, substrate is added, which reacts with the bound conjugate to produce blue color. More blue color means less aflatoxin. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of aflatoxin.

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