Veratox® MAX for Total Aflatoxin

Item No. 8035

  • Aqueous extraction - no harsh solvents
  • Detects B1, B2, G1 and G2 aflatoxin
  • Cost effective microwell format
  • Fast, accurate, and easy to use
BRAND Neogen Co.
MADE IN USA
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PRODUCT DETAILS

Veratox® MAX for Total Aflatoxin is intended for the quantitative analysis of aflatoxin in corn and corn gluten meal. This kit is designed to detect the four principle types of aflatoxin: B1, B2, G1 and G2 with superior cross reactivity when compared to other available kits. Veratox for Total Aflatoxin utilized an aqueous extraction procedure eliminating the need for harsh solvents in your laboratory.

Specifications

Analyte Aflatoxin
Approvals FGIS 2016-088
Brand Veratox®
Cross Reactivity Total aflatoxins (B1, B2, G1, G2)
Limit of Detection 2.5 ppb
Package Dimensions 7.40 IN x 4.00 IN x 4.90 IN
Package Weight 1.30 LB
Platform ELISA
Quantity per Package 40 tests
Range of Quantitation 5.00 ppb - 50.00 ppb
Result Type Quantitative
Testing Time 10.00 Minutes

  1. 48 antibody-coated microwells
  2. 48 red-marked mixing wells
  3. 4 yellow-labeled bottles of 0, 5, 15 and 50 ppb aflatoxin controls
  4. 1 blue-labeled bottle of aflatoxin-HRP conjugate solution
  5. 1 green-labeled bottle of K-Blue® Substrate solution
  6. 1 red-labeled bottle of Red Stop solution
  7. 25 MAX 2 Aqueous Extraction Packets
  8. 1 pink-labeled bottle of dilution diluent

  1. Extraction materials
    1. MAX 2 Aqueous Extraction Packets (Neogen item 8036)
    2. MAX 2 GIPSA Aqueous Extraction Packets (Neogen item 8036G)
    3. 250 mL graduated cylinder or dispensing pump (Neogen item 9368, 9448)
    4. Container with 125 mL capacity (Neogen item 9428)
    5. Filter syringes, Whatman #4 filter paper, or equivalent (Neogen item 9420, 9519, 9429)
    6. Sample collection tubes (Neogen item 9421)
  2. Agri-Grind grinder or equivalent (Neogen item 9401, 9453)
  3. Scale capable of weighing 5-50 grams (Neogen item 9427)
  4. Microwell reader with a 650 nm filter (Neogen item 9303)
  5. Pipettor, 12-channel (Neogen item 9273)
  6. Pipettor, 100 µL (Neogen item 9272, 9290)
  7. Pipette tips for 100 µL and 12-channel pipettors (Neogen item 9410, 9407, 9417)
  8. Paper towels or equivalent absorbent material
  9. Plastic bucket for use as waste receptacle
  10. Microwell holder (Neogen item 9402)
  11. Timer (Neogen item 9426)
  12. Waterproof marker
  13. Wash bottle (Neogen item 9400)
  14. 2 reagent boats for 12-channel pipettor (Neogen item 9450)
  15. Distilled or deionized water
  16. Shaker, Rockit (Neogen item 9390)
  17. Blender (Neogen item 9493)
  18. Blender jar, mini (Neogen item 9477)

Assay Principle

Veratox MAX for Total Aflatoxin is a direct competitive ELISA in a microwell format which allows the user to obtain exact concentrations in parts per billion (ppb). Free aflatoxin in the samples and controls is allowed to compete with enzyme-labeled aflatoxin (conjugate) for the antibody binding sites. After a wash step, substrate is added, which reacts with the bound conjugate to produce blue color. More blue color means less aflatoxin. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of aflatoxin.

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