YM-11 Agar

Dehydrate Powder Media
Item No. 6904A

BRAND Neogen Co.
MADE IN USA
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PRODUCT DETAILS

YM-11 Agar is used for the selective enumeration of yeasts and molds using the ISO-GRID® and/or NEO-GRID® Membrane Filtration System. YM-11 Agar is not intended for use in the diagnosis of disease or other conditions in humans. YM-11 Agar was formulated to stimulate rapid growth and colonial development of a wide range of fungi. Peptone sources were selected to maximize growth is this medium. YM-11 Agar is buffered at a neutral pH, and selective agents were included to inhibit bacteria growth. YM-11 Agar is recommended for the rapid enumeration of yeasts and molds in all foods using the ISO-GRID and/or NEO-GRID Membrane Filtration System method.

Specifications
Brand NEOGEN Culture Media
Formula
Formula Liter
Enzymatic Digest of Soybean Meal 20 g
Enzymatic Digest of Casein 20 g
Dextrose 5 g
Sodium Chloride 5 g
Potassium Phosphate, Dibasic 2.4 g
Trypan Blue 0.03 g
Chloramphenoicol 0.1 g
Agar 15 g
Mass 500 g
Package Dimensions 3.60 IN x 3.60 IN x 6.60 IN
Package Weight 1.30 LB

Preparation

  1. Suspend 67.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121° C for 15 minutes.
  4. Cool to 45 – 50°C. Aseptically add 20 mL of a 0.5 A% (wt/v) aqueous solution of Chlortetracycline HCl. Mix thoroughly.
  5. Check pH and adjust, if necessary to obtain a final pH of 7.0 ± 0.2 in the solidified medium.
  6. Dispense 18 – 20 mL volumes to sterile Petri dishes, cool until plates are solidified.

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