Reveal® for Peanut

Item No. 8438

  • Easy-to-use lateral flow format
  • Rapid and accurate screening test
  • Minimal equipment required
BRAND Neogen Co.
MADE IN USA
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PRODUCT DETAILS

Reveal® for Peanut Allergen is intended for the qualitative analysis of peanut residue in food products (e.g., cookies, crackers, chocolate bars, ice cream and cereals), clean-in-place rinses, and on environmental surfaces.

Specifications

Analyte Peanut
Brand Reveal®
Limit of Detection Screens samples at 5 ppm peanut
Package Dimensions 8.40 IN x 7.90 IN x 3.40 IN
Package Weight 0.90 LB
Platform Lateral Flow
Quantity per Package 25 tests
Result Type Qualitative
Testing Time 10.00 Minutes

  1. 25 lateral flow test strips in a sealed stay-dry tube
  2. 25 transfer pipettes
  3. 25 sample tubes
  4. 4 foil pouches of 10 mM PBS dry powder extraction solvent; each pouch contains enough powder to prepare 1 L of extraction solution
  5. 2 bottles of 50 g extraction additive
  6. Plastic 3 g scoop to measure extraction additive

  1. High-speed blender with a 250 mL jar (Neogen® item #9493/#9477)
  2. Allergen Extraction Kit (Neogen item #8429)
  3. Allergen Environmental Swabbing Kit (Neogen item #8432S)
  4. Scale capable of weighing 5 ± 0.1 grams (Neogen item #9427)
  5. Water bath, hot plate or equivalent heat source capable of maintaining 60°C ± 1°
  6. Thermometer
  7. 1 L bottle to prepare extraction solution (Neogen item #9472)
  8. Waterproof marker
  9. Distilled or deionized water
  10. Timer (Neogen item #9426)
  11. Graduated cylinder capable of measuring 125 mL (Neogen item #9368)
  12. Whatman #4 filter paper (Neogen item #9429)
  13. Reveal AccuScan® III System (Neogen item #9590) optional

Assay Principle

Reveal for Peanut Allergen is a single-step lateral flow immunochromatographic assay. The extract is wicked through a reagent zone, which contains antibodies specific for peanut protein conjugated to colored particles. If peanut is present, it will be captured by the conjugated antibodies. The peanut-antibody-particle complex is then wicked onto a membrane which contains a zone of antibody specific for peanut protein. This zone captures the complex allowing the particles to concentrate and form a visible line. If no peanut is present, no line will form. The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of peanut, ensuring the strip is working properly.

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