Veratox® for Gliadin

Item No. 8480

Accurate and easy-to-use
Cost effective microwell format
High specificity

BRAND	Neogen Co.
MADE IN	USA
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PRODUCT DETAILS

Veratox for Gliadin is used for the quantitative analysis of prolamins (wheat gliadin, rye secalin, and barley hordein) in ingredients, environmental surfaces, clean-in-place rinses, and finished food products intended to be prolamins (gluten) free. Can also be used to screen environmental swabs.

Specifications
Analyte	Gliadin/Gluten
Brand	Veratox®
Limit of Detection	5 ppm gliadin
Package Dimensions	10.50 IN x 8.00 IN x 2.80 IN
Package Weight	0.80 LB
Platform	ELISA
Prop 65 Warning	WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package	36 tests
Range of Quantitation	5.00 ppm gliadin - 50.00 ppm gliadin
Result Type	Quantitative
Testing Time	30.00 Minutes

PROCEDURE

Veratox for Gliadin is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Gliadin is extracted from samples with a 40% ethanol solution by shaking in a shaker or rotator. Extract is diluted in Phosphate Buffered Saline (PBS) and diluted samples are added to antibody-coated wells (capture antibody) where gliadin will bind to the antibody during an incubation period. Any unbound gliadin is washed away and a second antibody, which is enzyme labeled (detector antibody) is added. The detector antibody binds to the gliadin during another incubation period. Unbound enzyme-labeled antibody is washed away and a one step substrate is added. Color develops as a result of the presence of bound-labeled antibody. A stopping reagent is added and the color of the solution is observed. Blue color indicates samples containing high levels of gliadin while purple or red samples contain little or no gliadin. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of gliadin in parts per million (ppm).

Materials Provided

48 antibody-coated microwells
48 red-marked transfer wells
5 yellow-labeled bottles of 0, 5, 10, 20, and 50 ppm gliadin (0, 10, 20, 40 and 100 ppm gluten) controls (0, 12.5, 25, 50, and 125 ng/mL gliadin)
2 blue-labeled bottles of enzyme-labeled antibody conjugate
1 green-labeled bottle of K-Blue^® Substrate
1 red-labeled bottle of Red Stop solution
40 mL of 10 mM PBS-Tween washing reagent in a wide mouth bottle. Each bottle can prepare 1 L in distilled or deionized water (pH 7.4)
1 foil pouch of 10 mM PBS dry powder containing enough powder to prepare 1 L of dilution solvent
50 g of extraction additive in 2 specimen cups
Plastic scoop to measure extraction additive

Materials Required But Not Provided

Orbital rotator or shaker to hold 50 cc centrifuge tubes for a 1 g sample, or shaker water bath with clamps adjusted to hold 125 mL (4 oz) extraction bottles for a 2 g sample
Oven or water bath adjustable to 50°C if analyzing heat-processed samples
Scale capable of weighing 1 ± 0.1 g (Neogen item 9427), or scale capable of weighing 0.25 ± 0.01 g if using gliadin cocktail solution (Neogen item 9395)
Pipettor, 50–200 μL adjustable (Neogen item 9276)
Pipettor, 12-channel (Neogen item 9273)
Pipette tips (Neogen item 9410, 9417, 9407)
Three 1 L bottles to prepare washing solution, extract solution, and sample extract dilution solution (Neogen item 9472)
Test tubes to perform sample extract dilution
Timer (Neogen item 8426, 9452)
Microwell reader with a 650 nm filter (Neogen item 9303)
Three reagent boats for 12-channel pipettor (Neogen item 9435)
Microwell strip holder (Neogen item 9402)
Wash bottle (Neogen item 9400)
Paper towels or equivalent absorbent material
Waterproof marker
Distilled or deionized water
Laboratory grade ethanol (190 proof)
Special extraction additive for dark chocolate, cocoa and tannin (Neogen item 8482)

If testing heat treated products:

Gliadin renaturing cocktail solution for heat processed samples (Neogen item 8483, 8483B, 8483S)

TRAINING

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.