Reveal for Peanut Allergen is a single-step lateral flow immunochromatographic assay. The extract is wicked through a reagent zone, which contains antibodies specific for peanut protein conjugated to colored particles. If peanut is present, it will be captured by the conjugated antibodies. The peanut-antibody-particle complex is then wicked onto a membrane which contains a zone of antibody specific for peanut protein. This zone captures the complex allowing the particles to concentrate and form a visible line. If no peanut is present, no line will form. The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of peanut, ensuring the strip is working properly.