Reveal® for Peanut

901041L-Reveal-3-D-for-Fistik-Alerjen-Test-Kiti-min

Item No. 8438

Easy-to-use lateral flow format
Rapid and accurate screening test
Minimal equipment required

BRAND	Neogen Co.
MADE IN	USA
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PRODUCT DETAILS

Reveal® for Peanut Allergen is intended for the qualitative analysis of peanut residue in food products (e.g., cookies, crackers, chocolate bars, ice cream and cereals), clean-in-place rinses, and on environmental surfaces.

Specifications


Analyte	Peanut
Brand	Reveal®
Limit of Detection	Screens samples at 5 ppm peanut
Package Dimensions	8.40 IN x 7.90 IN x 3.40 IN
Package Weight	0.90 LB
Platform	Lateral Flow
Quantity per Package	25 tests
Result Type	Qualitative
Testing Time	10.00 Minutes

MATERIALS PROVIDED

25 lateral flow test strips in a sealed stay-dry tube
25 transfer pipettes
25 sample tubes
4 foil pouches of 10 mM PBS dry powder extraction solvent; each pouch contains enough powder to prepare 1 L of extraction solution
2 bottles of 50 g extraction additive
Plastic 3 g scoop to measure extraction additive

MATERIALS REQUIRED BUT NOT PROVIDED

High-speed blender with a 250 mL jar (Neogen^® item #9493/#9477)
Allergen Extraction Kit (Neogen item #8429)
Allergen Environmental Swabbing Kit (Neogen item #8432S)
Scale capable of weighing 5 ± 0.1 grams (Neogen item #9427)
Water bath, hot plate or equivalent heat source capable of maintaining 60°C ± 1°
Thermometer
1 L bottle to prepare extraction solution (Neogen item #9472)
Waterproof marker
Distilled or deionized water
Timer (Neogen item #9426)
Graduated cylinder capable of measuring 125 mL (Neogen item #9368)
Whatman #4 filter paper (Neogen item #9429)
Reveal AccuScan^® III System (Neogen item #9590) optional

Assay Principle

Reveal for Peanut Allergen is a single-step lateral flow immunochromatographic assay. The extract is wicked through a reagent zone, which contains antibodies specific for peanut protein conjugated to colored particles. If peanut is present, it will be captured by the conjugated antibodies. The peanut-antibody-particle complex is then wicked onto a membrane which contains a zone of antibody specific for peanut protein. This zone captures the complex allowing the particles to concentrate and form a visible line. If no peanut is present, no line will form. The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of peanut, ensuring the strip is working properly.