Veratox® for Casein

8460-Veratox-for-Kazein-Alerjen-Test-Kiti-min

Item No. 8460

Accurate and easy-to-use
Cost effective microwell format
High specificity

BRAND	Neogen Co.
MADE IN	USA
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PRODUCT DETAILS

Veratox® for Casein Allergen is used for the quantitative analysis of casein residue in food products such as juices, wine, sauces, sorbets, or clean-in-place rinses. Can also be used to screen environmental swabs.

Specifications


Analyte	Casein, Milk
Brand	Veratox®
Limit of Detection	1 ppm casein (NFDM scale)
Package Dimensions	10.50 IN x 8.00 IN x 2.70 IN
Package Weight	0.70 LB
Platform	ELISA
Quantity per Package	38 tests
Range of Quantitation	2.50 ppm casein - 15.00 ppm casein
Result Type	Quantitative
Testing Time	30.00 Minutes

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.

Materials Provided

48 antibody-coated microwells (24 per pouch)
48 red-marked transfer wells (24 per pouch)
5 yellow-labeled bottles of 0, 2.5, 5, 10, 15 ppm nonfat dry milk (NFDM) controls
4 blue-labeled bottles of enzyme-labeled antibody conjugate
1 green-labeled bottle of K-Blue^® Substrate
1 red-labeled bottle of Red Stop solution
5 foil pouches of 10 mM PBS dry powder extraction solvent (each pouch contains enough powder to prepare 1 L of extraction solvent)
2 widemouth bottles of 40 mL PBS-Tween wash buffer concentrate (each bottle contains enough concentrate to prepare 1 L of wash buffer)
50 g of extraction additive in a specimen cup
Plastic scoop to measure extraction additive

Materials Required But Not Provided

Allergen Extraction Kit (Neogen^® item 8429)
1. 20 disposable plastic extraction bottles
2. 20 disposable transfer pipettes
Shaker water bath adjusted to 60°C ± 1° (140°F) with clamps to hold extraction bottles
Adjustable 50-200 µL pipettor (Neogen item 9276)
Pipette tips (Neogen item 9410)
12-channel pipettor (Neogen item 9273)
Timer (Neogen item 9426)
Microwell strip reader with a 650 nm filter (Neogen item 9303)
1 L bottle to prepare washing solution (Neogen item 9472)
1 L heat safe bottle to prepare extract solution (Neogen item 9472)
Paper towels or equivalent absorbent material
Microwell holder (Neogen item 9402)
Waterproof marker
Wash bottle (Neogen item 9400)
Distilled or deionized water
3 reagent multichannel pipettor boats (Neogen item 9435)
Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
Scale capable of weighing 5 g (Neogen item 9435)

Procedures

The Veratox for Casein Allergen test is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Casein residue is extracted from samples with a buffered salt solution (Phosphate Buffered Saline, or PBS) by shaking in a heated water bath. Extracted casein residue is sampled and added to antibody-coated wells (capture antibody) where it binds to the antibody during an incubation. Any unbound residue is washed away and a second antibody (detector antibody), which is enzyme labeled, is added. The detector antibody binds to the already bound casein residue. After a second wash, the substrate is added. Color develops as a result of the presence of bound detector antibody. Red Stop reagent is added and the color of the resulting solution is observed. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of allergen.