Veratox® for Mustard – ABP | Grain, Flour, Food and Feed Quality Control Devices

Veratox for Mustard

Item No. 8400

Accurate and easy-to-use
Quantitate or screen samples
High specificity

BRAND	Neogen Co.
MADE IN	USA
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PRODUCT DETAILS

Veratox® for Mustard Allergen is used for the quantitative analysis of mustard proteins in food products such as spices, sauces, dressings, meats and clean-in-place rinses. Can also be used to screen environmental swabs.

Specifications
Analyte	Mustard
Brand	Veratox®
Limit of Detection	<1 ppm mustard
Package Dimensions	10.50 IN x 8.00 IN x 2.75 IN
Package Weight	0.90 LB
Platform	ELISA
Prop 65 Warning	WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package	38 tests
Range of Quantitation	2.50 ppm mustard - 25.00 ppm mustard
Result Type	Quantitative
Testing Time	30.00 Minutes

PROCEDURE

The Veratox for Mustard Allergen test is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Mustard protein residue is extracted from samples with a Tris-EDTA solution by shaking in a heated water bath, followed by centrifugation or filtration. Extracted mustard protein is sampled and added to antibody-coated wells (capture antibody) where it binds to the antibody during an incubation. Any unbound mustard protein is washed away and a second antibody (detector antibody), which is enzyme labeled, is added. The detector antibody binds to the already bound mustard protein. After a second wash, substrate is added. Color develops as a result of the presence of bound detector antibody. Red Stop reagent is added and the color of the resulting solution is observed. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of mustard, expressed as ppm mustard.

Materials Provided

48 antibody-coated microwells (24 per pouch)
48 red-marked transfer wells (24 per pouch)
5 yellow-labeled bottles of 0, 2.5, 5, 10, 25 ppm mustard controls
2 blue-labeled bottles of enzyme-labeled antibody conjugate
1 green-labeled bottle of K-Blue^® Substrate
1 red-labeled bottle of Red Stop solution
5 pouches of Veratox mustard allergen extraction buffer. Each pouch is enough to prepare 1 L in distilled or deionized water.
40 mL of 10 mM PBS-Tween washing reagent in a wide mouth bottle. Each bottle is enough to prepare 1 L in distilled or deionized water (pH 7.4)
50 g of extraction additive in a specimen cup
Plastic scoop to measure extraction additive

Materials Required But Not Provided

Allergen Extraction Kit (Neogen^® item 8429)
1. 20 disposable plastic extraction bottles
2. 20 disposable transfer pipettes
Allergen Environmental Swabbing Kit (Neogen item 8432S)
1. 100 sterile sawbs
2. 100 dropper tips
Shaker water bath capable of maintaining 60 ± 1°C with clamps to hold 250 mL extraction bottles
Whatman no. 4 filters or equivalent (Neogen item 9429)
Centrifuge (optional)
Filter funnel, 65 mm (Neogen item 9353)
Pipettor, 50–200 µL, adjustable (Neogen item 9276)
Pipettor, 12-channel (Neogen item 9273)
Pipette tips (Neogen item 9410, 9407, 9417)
Timer (Neogen item 9426)
Microwell reader with a 650 nm filter (Neogen item 9303)
Bottle, 1 L, to prepare washing solution (Neogen item 9472)
Bottle, 1 L heat safe, to prepare extract solution (Neogen item 9472)
Paper towels or equivalent absorbent material
Microwell holder (Neogen item 9402)
Waterproof marker
Wash bottle (Neogen item 9400)
Distilled or deionized water
3 reagent boats for 12-channel pipettor (Neogen item 9435)
Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
Scale capable of weighing 5 ± 0.1 g (Neogen item 9427)

TRAINING

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.