Reveal® for Histamine

Item No. 9501

  • Simple water extraction
  • Rapid 5 minutes test time
BRAND Neogen Co.
MADE IN USA
SHARE

PRODUCT DETAILS

Reveal® for Histamine is a single-step lateral flow assay based on a competitive immunoassay format intended for the visual screening of histamine in scrombroid species of fish, such as tuna and mahi-mahi.

Specifications
Analyte Histamine
Brand Reveal®
Cross Reactivity Specific for histamine. No cross-reactivity with other biogenic amines.
Limit of Detection 50 ppm
Package Dimensions 8.30 IN x 5.10 IN x 2.50 IN
Package Weight 1.10 LB
Platform Lateral Flow
Quantity per Package 25 tests
Result Type Qualitative
Testing Time 5.00 Minutes

Assay Principle

Reveal for Histamine is a single-step lateral flow assay based on a competitive immunoassay format. The extract is wicked through a reagent zone, which contains histamine-specific antibodies conjugated to colored particles. If histamine is present, it will be captured by the particle-antibody complex. The histamine-antibody-particle complex then is wicked onto a membrane, which contains a zone of histamine conjugated to a protein carrier. This zone captures only uncomplexed antibody-particle conjugate, allowing the colored particles to concentrate and form a visible line. The membrane also contains a control zone where the antibody-particle complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of histamine, ensuring the strip is functioning properly. In addition, the control line also is used as a reference for comparison to the test zone for visual interpretation of the test results. If histamine is present above a pre-determined limit, a line lighter than the control line or no line will form. Therefore, a positive sample will result in a light line or absence of a line in the test zone. Alternatively, a negative sample will form a line of the same or darker intensity as the control line in the test zone.

Materials Provided

  1. 25 histamine test strips
  2. 25 sample cups
  3. 25 bottles of 7 mL sample diluent

Material Required But Not Provided

  1. Graduated cylinder (Neogen® item 9368)
  2. Sample extraction bottles, 250 mL (Neogen item 9399)
  3. Sample collection tubes (Neogen item 9421)
  4. Blender (Neogen item 9493, 9477)
  5. Scale capable of weighing 10–50 grams (Neogen item 9427)
  6. Pipettor, 100 µL (Neogen items 9272, 9278, 9276)
  7. Pipette tips (Neogen items 9410, 9407, 9417)
  8. Paper towels or equivalent absorbent material
  9. Rack for Reveal sample cups (Neogen item 9475)
  10. Timer (Neogen item 9426)
  11. Waterproof marker
  12. Distilled or deionized water
  13. AccuScan reader system (Neogen item 9565) (Optional)

Training

Our customers’ success is our shared success.

Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.

Aflatoxin Test Kits

Doksinivalenol (DON) Test Kits

Fumonisin Test Kits

Ochratoxin Test Kits

T-2/HT-2 Test Kits

Zearalenone Test Kits

AccuClean Advance

Pestisit Test Kits

CP4 Roundup Ready GDO Test Kits

Histamine Test Kits

Crustacea Test Kits

Biokits Test Kits

Pathogen Test Kits

Allergen Test Kits

Hygiene & Sanitation Control

Sulfide Test Kits

REQUEST FORM

Name Surname*
Company
Phone*
E-mail
City*
Product / Service*
Your Message
TOP
×

Welcome to ABP WhatsApp Line ...

You can contact us and get information about our products and services...

× WHATSAPP